In Vitro Propagation and Histochemical Analysis of Launaea sarmentosa (Willd.) Kuntze

Launaea sarmentosa (Willd.) Kuntze., an edible perennial herb, is critically considered endangered species due to its habitat destruction. In vitro L. propagation was therefore conducted preserve this plant. Two types of explants including leaf and stolon were applied induce the multiple shoots by culturing on Murashige Skoog (MS) medium supplemented with BA (0, 1, 2 mg/L) 2,4-D 2, 4 mg/L). The optimal shoot induction for both MS containing mg/l (presented 100 % induction). Roots induced various concentrations auxins [1-naphthalene acetic acid (NAA), indole-3-acetic (IAA), indole-3-butyric (IBA)]. highest percentage rooting (70.00 ± 11.05 %) obtained from stolon-derived 0.1 IAA. One-hundred percent survivability presented after transforming plantlets into a mixture soil, sand, peat moss (1:1:1). Starch, protein, lipid accumulated in leaves detected histochemical technique. information will be useful tool mass obtain basic knowledge plant accumulating substances. HIGHLIGHTS This paper highlights explant stolons have great potential produce inoculation adding BA. All regenerated plants get success acclimatization transplantation greenhouse. fat, protein carbohydrate which might related precursor medicinal properties GRAPHICAL ABSTRACT